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The Importance of SOXC in Regulating Adult Bone Mass – A Study in Nature Communications

A recent study published in Nature Communications has shed light on the crucial role of the transcription factor SOXC in...

Colorectal cancer is one of the most common types of cancer worldwide, with over 1.8 million new cases diagnosed each...

Colorectal cancer is one of the most common types of cancer worldwide, with over 1.8 million new cases diagnosed each...

A recent study published in npj Regenerative Medicine has shed light on how the adult environment plays a crucial role...

A recent study published in Nature Communications has shed light on the role of mesenchymal glioma stem cells in inducing...

A recent study published in Nature Cancer has shed light on the role of threonine in promoting brain tumor growth...

Heterochromatin, H3.3, and Mesoblast are three key components in the field of molecular biology that have been the focus of...

Bioengineering vascularized porcine flaps through perfusion-recellularization is a cutting-edge technique that holds great promise for the field of regenerative medicine....

As we age, our brains undergo a series of changes that can impact our cognitive function and overall well-being. One...

Microplastics have become a major environmental concern in recent years, as they have been found to have detrimental effects on...

A recent study published in Nature Cell Biology has shed light on the role of Cyclophilin A in supporting the...

A groundbreaking study published in Nature Aging has shed light on a potential new approach to restoring neuronal progenitors in...

The Regenexx Stem Cell Institute of America, a prominent provider of stem cell therapy, has recently been found guilty in...

A groundbreaking study published in Nature Cell Biology has revealed new insights into the process of direct neurogenesis in the...

The Georgia Attorney General and the Federal Trade Commission have successfully prosecuted Superior Healthcare, a stem cell clinic, for deceptive...

Ovarian cancer is a deadly disease that affects thousands of women each year. Traditional treatments such as surgery, chemotherapy, and...

Aging is a natural process that affects all living organisms, including humans. As we age, our immune system undergoes changes...

Knee osteoarthritis (OA) is a common and debilitating condition that affects millions of people worldwide. It is characterized by the...

Spinal cord injury (SCI) is a devastating condition that can result in permanent paralysis and loss of function. While there...

Spinal cord injury (SCI) is a devastating condition that can result in permanent paralysis and loss of function. Current treatments...

Autophagy, the process by which cells break down and recycle their own components, plays a crucial role in the development...

Autophagy is a crucial cellular process that plays a key role in maintaining cellular homeostasis by degrading and recycling damaged...

The California Institute for Regenerative Medicine (CIRM) has recently announced funding for a groundbreaking clinical research project focused on personalized...

Ischaemic bowel disease, also known as mesenteric ischaemia, is a condition characterized by reduced blood flow to the intestines, leading...

Allogeneic transplant-associated thrombotic microangiopathy (TA-TMA) is a serious complication that can occur in patients who have undergone allogeneic stem cell...

Clostridioides difficile infection (CDI) is a common and potentially life-threatening complication in patients undergoing hematopoietic stem cell transplantation (HSCT). CDI...

In the ever-evolving world of science and technology, there are constant breakthroughs and advancements being made in the field of...

In this week’s edition of The Niche, we delve into the exciting world of anti-aging techniques, stem cell research, and...

SOX9 is a critical transcription factor that plays a key role in the transition from tissue regeneration to fibrosis. This...

SOX9 is a critical transcription factor that plays a key role in various biological processes, including development, differentiation, and tissue...

How to Produce Standardized hPSC-Derived Cardiomyocyte Aggregates in Stirred Spinner Flasks: A Guide from Nature Protocols

Producing standardized human pluripotent stem cell (hPSC)-derived cardiomyocyte aggregates in stirred spinner flasks is a crucial step in the field of regenerative medicine and drug discovery. These aggregates, also known as cardiac spheroids, mimic the three-dimensional structure and function of the heart, making them an invaluable tool for studying cardiac development, disease modeling, and drug screening. In a recent article published in Nature Protocols, researchers have outlined a detailed protocol for generating high-quality hPSC-derived cardiomyocyte aggregates in stirred spinner flasks. Here, we will provide an overview of the key steps involved in this protocol.

First and foremost, it is essential to start with high-quality hPSCs that have been properly maintained and characterized. These cells should be cultured in a pluripotent state using appropriate culture media and conditions. Once the hPSCs are ready, they can be differentiated into cardiomyocytes using a well-established cardiac differentiation protocol. This typically involves the sequential addition of growth factors and small molecules that mimic the signaling pathways involved in cardiac development.

After the cardiomyocytes have been generated, they can be seeded into stirred spinner flasks for aggregate formation. The use of stirred spinner flasks allows for efficient mixing and oxygenation of the cells, which is crucial for promoting uniform aggregate formation. The researchers recommend using a specific type of spinner flask with a magnetic stir bar for optimal results.

To promote aggregation, the cardiomyocytes are typically cultured in a suspension culture medium that contains essential nutrients and growth factors. The researchers suggest using a defined medium that supports cardiomyocyte survival and maturation. It is also important to monitor the pH and oxygen levels in the culture medium to ensure optimal conditions for aggregate formation.

During the culture period, the researchers recommend gently shaking the spinner flasks at a low speed to promote aggregate formation without disrupting the cells. It is also important to regularly check the size and morphology of the aggregates under a microscope to ensure uniformity and quality. The researchers provide guidelines for determining the optimal seeding density and culture duration to achieve standardized cardiomyocyte aggregates.

In conclusion, producing standardized hPSC-derived cardiomyocyte aggregates in stirred spinner flasks is a complex process that requires careful attention to detail and adherence to specific protocols. By following the guidelines outlined in the Nature Protocols article, researchers can generate high-quality cardiac spheroids that can be used for a wide range of applications in cardiovascular research. This protocol represents a significant advancement in the field of stem cell biology and holds great promise for advancing our understanding of cardiac development and disease.