Boisset, J. C. et al. In vivo imaging of haematopoietic cells emerging from the mouse aortic endothelium. Nature 464, 116–120 (2010).
de Bruijn, M. F. et al. Hematopoietic stem cells localize to the endothelial cell layer in the midgestation mouse aorta. Immunity 16, 673–683 (2002).
Taoudi, S. & Medvinsky, A. Functional identification of the hematopoietic stem cell niche in the ventral domain of the embryonic dorsal aorta. Proc. Natl Acad. Sci. USA 104, 9399–9403 (2007).
Mascarenhas, M. I., Parker, A., Dzierzak, E. & Ottersbach, K. Identification of novel regulators of hematopoietic stem cell development through refinement of stem cell localization and expression profiling. Blood 114, 4645–4653 (2009).
Yokomizo, T. & Dzierzak, E. Three-dimensional cartography of hematopoietic clusters in the vasculature of whole mouse embryos. Development 137, 3651–3661 (2010).
Kumaravelu, P. et al. Quantitative developmental anatomy of definitive haematopoietic stem cells/long-term repopulating units (HSC/RUs): role of the aorta-gonad-mesonephros (AGM) region and the yolk sac in colonisation of the mouse embryonic liver. Development 129, 4891–4899 (2002).
Boisset, J. C. et al. Progressive maturation toward hematopoietic stem cells in the mouse embryo aorta. Blood 125, 465–469 (2015).
Patel, S. H. et al. Lifelong multilineage contribution by embryonic-born blood progenitors. Nature 606, 747–753 (2022).
Yokomizo, T. et al. Independent origins of fetal liver haematopoietic stem and progenitor cells. Nature 609, 779–784 (2022).
Ganuza, M. et al. Lifelong haematopoiesis is established by hundreds of precursors throughout mammalian ontogeny. Nat. Cell Biol. 19, 1153–1163 (2017).
Thambyrajah, R. et al. GFI1 proteins orchestrate the emergence of haematopoietic stem cells through recruitment of LSD1. Nat. Cell Biol. 18, 21–32 (2016). ncb3276 [pii].
Kauts, M. L. et al. In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells Corresponds to In Vivo Waves of Hematopoietic Cell Generation. Stem Cell Rep. 10, 151–165 (2018). S2213-6711(17)30526-X [pii].
Nottingham, W. T. et al. Runx1-mediated hematopoietic stem-cell emergence is controlled by a Gata/Ets/SCL-regulated enhancer. Blood 110, 4188–4197 (2007). blood-2007-07-100883 [pii].
Eich, C. et al. In vivo single cell analysis reveals Gata2 dynamics in cells transitioning to hematopoietic fate. J. Exp. Med. 215, 233–248 (2018). jem.20170807 [pii].
Chen, M. J., Yokomizo, T., Zeigler, B. M., Dzierzak, E. & Speck, N. A. Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter. Nature 457, 887–891 (2009).
Vink, C. S. et al. Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells. Cell Rep 31. (2020).
Porcheri, C. et al. Notch ligand Dll4 impairs cell recruitment to aortic clusters and limits blood stem cell generation. EMBO J. 39, e104270 (2020).
Fadlullah, M. Z. et al. Murine AGM single-cell profiling identifies a continuum of hemogenic endothelium differentiation marked by ACE. Blood. blood.2020007885 [pii] https://doi.org/10.1182/blood.2020007885 S0006-4971(21)01595-0 [pii] (2021).
Lancrin, C. et al. GFI1 and GFI1B control the loss of endothelial identity of hemogenic endothelium during hematopoietic commitment. Blood 120, 314–322 (2012).
Bertrand, J. Y. et al. Haematopoietic stem cells derive directly from aortic endothelium during development. Nature 464, 108–111 (2010).
Kissa, K. & Herbomel, P. Blood stem cells emerge from aortic endothelium by a novel type of cell transition. Nature 464, 112–115 (2010).
Medvinsky, A., Rybtsov, S. & Taoudi, S. Embryonic origin of the adult hematopoietic system: advances and questions. Development 138, 1017–1031 (2011).
Rybtsov, S. et al. Hierarchical organization and early hematopoietic specification of the developing HSC lineage in the AGM region. J. Exp. Med. 208, 1305–1315 (2011).
Taoudi, S. et al. Extensive hematopoietic stem cell generation in the AGM region via maturation of VE-cadherin+CD45+ pre-definitive HSCs. Cell Stem Cell 3, 99–108 (2008).
Zhou, F. et al. Tracing haematopoietic stem cell formation at single-cell resolution. Nature 533, 487–492 (2016).
Hadland, B. K., Varnum-Finney, B., Nourigat-Mckay, C., Flowers, D. & Bernstein, I. D. Clonal analysis of embryonic hematopoietic stem cell precursors using single cell index sorting combined with endothelial cell niche co-culture. J. Vis. Exp. 2018. https://doi.org/10.3791/56973 (2018).
Baron, C. S. et al. Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta. Nat. Commun. 9, 2517 (2018).
Zhu, Q. et al. Developmental trajectory of prehematopoietic stem cell formation from endothelium. Blood 136, 845–856 (2020).
Artavanis-Tsakonas, S., Rand, M. D. & Lake, R. J. Notch signaling: cell fate control and signal integration in development. Science 284, 770–776 (1999).
Gazave, E. et al. Origin and evolution of the Notch signalling pathway: An overview from eukaryotic genomes. Preprint, https://doi.org/10.1186/1471-2148-9-249 (2009).
Kageyama, R., Ohtsuka, T. & Kobayashi, T. The Hes gene family: Repressors and oscillators that orchestrate embryogenesis. Development 134, 1243–1251 (2007).
Bray, S. J. Notch signalling in context. Preprint, https://doi.org/10.1038/nrm.2016.94 (2016).
Gozlan, O. & Sprinzak, D. Notch signaling in development and homeostasis. Development 150, dev201138 (2023).
Nandagopal, N., Santat, L. A. & Elowitz, M. B. Cis-activation in the Notch signaling pathway. Elife 8. https://doi.org/10.7554/eLife.37880 (2019).
Sprinzak, D. et al. Cis-interactions between Notch and Delta generate mutually exclusive signalling states. Nature 465, 86–90 (2010).
Del Álamo, D., Rouault, H. & Schweisguth, F. Mechanism and significance of cis-inhibition in notch signalling. Preprint, https://doi.org/10.1016/j.cub.2010.10.034 (2011).
Xu, X. et al. Jag1-Notch cis-interaction determines cell fate segregation in pancreatic development. Nat. Commun. 14, 348 (2023).
Takeuchi, H., and Haltiwanger, R. S. Significance of glycosylation in Notch signaling. Preprint, https://doi.org/10.1016/j.bbrc.2014.05.115. (2014).
LeBon, L., Lee, T. V., Sprinzak, D., Jafar-Nejad, H. & Elowitz, M. B. Fringe proteins modulate Notch-ligand cis and trans interactions to specify signaling states. Elife 3, e02950 (2014).
Thambyrajah, R. & Bigas, A. Notch Signaling in HSC Emergence: When, Why and How. Cells 11, 358 (2022).
Hadland, B. K. et al. A requirement for Notch1 distinguishes 2 phases of definitive hematopoiesis during development. Blood 104, 3097–3105 (2004).
Kumano, K. et al. Notch1 but not Notch2 is essential for generating hematopoietic stem cells from endothelial cells. Immunity 18, 699–711 (2003).
Gama-Norton, L. et al. Notch signal strength controls cell fate in the haemogenic endothelium. Nat. Commun. 6. https://doi.org/10.1038/ncomms9510 (2015).
Robert-Moreno, À. et al. Impaired embryonic haematopoiesis yet normal arterial development in the absence of the Notch ligand Jagged1. EMBO J. 27, 1886–1895 (2008).
Laruy, B., Garcia-Gonzalez, I., Casquero-Garcia, V., and Benedito, R. Endothelial-to-hematopoietic transition is induced by Notch glycosylation and upregulation of Mycn. bioRxiv, 2020.09.13.295238. https://doi.org/10.1101/2020.09.13.295238 (2020).
Souilhol, C. et al. Developing HSCs become Notch independent by the end of maturation in the AGM region. Blood 128, 1567–1577 (2016).
Kieusseian, A., de la Grange, P. B., Burlen-Defranoux, O., Godin, I., and Cumano, A. Immature hematopoietic stem cells undergo maturation in the fetal liver. Development (Cambridge) 139. https://doi.org/10.1242/dev.079210 (2012).
Cumano, A., Ferraz, J. C., Klaine, M., Di Santo, J. P. & Godin, I. Intraembryonic, but not yolk sac hematopoietic precursors, isolated before circulation, provide long-term multilineage reconstitution. Immunity 15, 477–485 (2001).
Robert-Moreno, À., Espinosa, L., de la Pompa, J. L. & Bigas, A. RBPjκ-dependent Notch function regulates Gata2 and is essential for the formation of intra-embryonic hematopoietic cells. Development 132, 1117–1126 (2005).
Burns, C. E., Traver, D., Mayhall, E., Shepard, J. L. & Zon, L. I. Hematopoietic stem cell fate is established by the Notch-Runx pathway. Genes Dev. 19, 2331–2342 (2005).
Guiu, J. et al. Hes repressors are essential regulators of hematopoietic stem cell development downstream of notch signaling. J. Exp. Med. 210, 71–84 (2013).
Singh, N., Phillips, R. A., Iscove, N. N. & Egan, S. E. Expression of notch receptors, notch ligands, and fringe genes in hematopoiesis. Exp. Hematol. 28, 527–534 (2000).
Duarte, A. et al. Dosage-sensitive requirement for mouse Dll4 in artery development. Genes Dev. 18, 2474–2478 (2004).
Krebs, L. T. et al. Haploinsufficient lethality and formation of arteriovenous malformations in Notch pathway mutants. Genes Dev. 18, 2469–2473 (2004).
Gale, N. W. et al. Haploinsufficiency of delta-like 4 ligand results in embryonic lethality due to major defects in arterial and vascular development. Proc. Natl Acad. Sci. USA 101, 15949–15954 (2004).
Krebs, L. T. et al. Notch signaling is essential for vascular morphogenesis in mice. Genes Dev. 14, 1343–1352 (2000).
Lizama, C. O. et al. Repression of arterial genes in hemogenic endothelium is sufficient for haematopoietic fate acquisition. Nat. Commun. 6, 7739 (2015).
Richard, C. et al. Endothelio-Mesenchymal Interaction Controls runx1 Expression and Modulates the notch Pathway to Initiate Aortic Hematopoiesis. Dev. Cell 24, 600–611 (2013).
Zhang, P. et al. G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition. Cell Res. 25, 1093–1107 (2015).
Song, Y., Kumar, V., Wei, H.-X., Qiu, J. & Stanley, P. Lunatic, Manic, and Radical Fringe Each Promote T and B Cell Development. J. Immunol. 196, 232–243 (2016).
Vizan, P. et al. The Polycomb-associated factor PHF19 controls hematopoietic stem cell state and differentiation. Sci. Adv. 6, eabb2745 (2020). abb2745 [pii].
Uenishi, G. I. et al. NOTCH signaling specifies arterial-type definitive hemogenic endothelium from human pluripotent stem cells. Nat. Commun. 9, 1828 (2018).
Dignum, T. et al. Multipotent progenitors and hematopoietic stem cells arise independently from hemogenic endothelium in the mouse embryo. Cell Rep. 36, 109675 (2021).
Sprinzak, D., Lakhanpal, A., LeBon, L., Garcia-Ojalvo, J. & Elowitz, M. B. Mutual inactivation of Notch receptors and ligands facilitates developmental patterning. PLoS Comput Biol. 7, e1002069 (2011).
Jacobsen, T. L., Brennan, K., Arias, A. M. & Muskavitch, M. A. T. Cis-interactions between Delta and Notch modulate neurogenic signalling in Drosophila. Development 125, 4531–4540 (1998).
De Celis, J. F. & Bray, S. Feed-back mechanisms affecting Notch activation at the dorsoventral boundary in the Drosophila wing. Development 124, 3241–3251 (1997).
Micchelli, C. A., Rulifson, E. J. & Blair, S. S. The function and regulation of cut expression on the wing margin of Drosophila: Notch, Wingless and a dominant negative role for Delta and Serrate. Development 124, 1485–1495 (1997).
Becam, I., Fiuza, U. M., Arias, A. M. & Milán, M. A Role of Receptor Notch in Ligand cis-Inhibition in Drosophila. Curr. Biol. 20, 554–560 (2010).
Fleming, R. J. et al. An extracellular region of Serrate is essential for ligandinduced cis-inhibition of Notch signaling. Development (Cambridge) 140. https://doi.org/10.1242/dev.087916 (2013).
Ayllon, V. et al. The Notch ligand DLL4 specifically marks human hematoendothelial progenitors and regulates their hematopoietic fate. Leukemia 29, 1741–1753 (2015).
Slukvin, I. I. Generating human hematopoietic stem cells in vitro -exploring endothelial to hematopoietic transition as a portal for stemness acquisition. FEBS Lett. 590, 4126–4143 (2016).
Jung, H. S. et al. SOX17 integrates HOXA and arterial programs in hemogenic endothelium to drive definitive lympho-myeloid hematopoiesis. Cell Rep. 34, 108758 (2021).
Park, M. A. et al. Activation of the Arterial Program Drives Development of Definitive Hemogenic Endothelium with Lymphoid Potential. Cell Rep. 23, 2467–2481 (2018).
Ditadi, A. et al. Human definitive haemogenic endothelium and arterial vascular endothelium represent distinct lineages. Nat. Cell Biol. 17, 580–591 (2015).
Lee, J. B. et al. Notch-HES1 signaling axis controls hemato-endothelial fate decisions of human embryonic and induced pluripotent stem cells. Blood 122, 1162–1173 (2013).
Wang, L. et al. Generation of hematopoietic repopulating cells from human embryonic stem cells independent of ectopic HOXB4 expression. J. Exp. Med. 201, 1603–1614 (2005).
Varnum-Finney, B., Brashem-Stein, C. & Bernstein, I. D. Combined effects of Notch signaling and cytokines induce a multiple log increase in precursors with lymphoid and myeloid reconstituting ability. Blood 101, 1784–1789 (2003).
Delaney, C., Varnum-Finney, B., Aoyama, K., Brashem-Stein, C. & Bernstein, I. D. Dose-dependent effects of the Notch ligand Delta1 on ex vivo differentiation and in vivo marrow repopulating ability of cord blood cells. Blood 106, 2693–2699 (2005).
Ohishi, K., Varnum-Finney, B. & Bernstein, I. D. Delta-1 enhances marrow and thymus repopulating ability of human CD34+CD38- cord blood cells. J. Clin. Investig. 110, 1165–1174 (2002).
Delaney, C. et al. Notch-mediated expansion of human cord blood progenitor cells capable of rapid myeloid reconstitution. Nat. Med 16, 232–236 (2010).
Suzuki, N. et al. Generation of engraftable hematopoietic stem cells from induced pluripotent stem cells by way of teratoma formation. Mol. Ther. 21, 1424–1431 (2013).
Amabile, G. et al. In vivo generation of transplantable human hematopoietic cells from induced pluripotent stem cells. Blood 121, 1255–1264 (2013).
Dobin, A. et al. STAR: ultrafast universal RNA-seq aligner. Bioinforma. (Oxf., Engl.) 29, 15–21 (2013).
Anders, S., Pyl, P. T. & Huber, W. HTSeq–a Python framework to work with high-throughput sequencing data. Bioinformatics 31, 166–169 (2015). Jan 15.
Scialdone, A. et al. Computational assignment of cell-cycle stage from single-cell transcriptome data. Methods 85, 54–61 (2015). Sep 1.
R Core Team. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. (2021).
Martin, M. Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet.Journal; Vol 17, No 1: Next Generation Sequencing Data AnalysisDO– https://doi.org/10.14806/Ej.17.1.200 (2011).
Liao, Y., Smyth, G. K. & Shi, W. featureCounts: an efficient general purpose program for assigning sequence reads to genomic features. Bioinforma. (Oxf., Engl.) 30, 923–930 (2014).
Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15, 550 (2014).
- SEO Powered Content & PR Distribution. Get Amplified Today.
- PlatoData.Network Vertical Generative Ai. Empower Yourself. Access Here.
- PlatoAiStream. Web3 Intelligence. Knowledge Amplified. Access Here.
- PlatoESG. Carbon, CleanTech, Energy, Environment, Solar, Waste Management. Access Here.
- PlatoHealth. Biotech and Clinical Trials Intelligence. Access Here.
- Source: https://www.nature.com/articles/s41467-024-45716-y