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A bioluminescent and homogeneous assay for monitoring GPCR-mediated cAMP modulation and PDE activity – Scientific Reports

  • Sutherland, E. W. & Rall, T. W. Fractionation and characterization of a cyclic adenine ribonucleotide formed by tissue particles. J. Biol. Chem. 232(2), 1077–1091 (1958).

    CAS 
    PubMed 

    Google Scholar
     

  • Yau, K. W. Cyclic nucleotide-gated channels: an expanding new family of ion channels. Proc Natl Acad Sci U S A 91(9), 3481–3483 (1994).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Houslay, M. D. & Milligan, G. Tailoring cAMP-signalling responses through isoform multiplicity. Trends Biochem. Sci. 22(6), 217–224 (1997).

    CAS 
    PubMed 

    Google Scholar
     

  • Francis, S. H., Blount, M. A. & Corbin, J. D. Mammalian cyclic nucleotide phosphodiesterases: Molecular mechanisms and physiological functions. Physiol. Rev. 91(2), 651–690 (2011).

    CAS 
    PubMed 

    Google Scholar
     

  • Taylor, S. S. et al. Structural framework for the protein kinase family. Annu Rev Cell Biol 8, 429–462 (1992).

    CAS 
    PubMed 

    Google Scholar
     

  • Bos, J. L. Epac: A new cAMP target and new avenues in cAMP research. Nat. Rev. Mol. Cell Biol. 4(9), 733–738 (2003).

    CAS 
    PubMed 

    Google Scholar
     

  • Zaccolo, M. & Pozzan, T. CAMP and Ca2+ interplay: A matter of oscillation patterns. Trends Neurosci. 26(2), 53–55 (2003).

    CAS 
    PubMed 

    Google Scholar
     

  • Brand, T. The Popeye domain-containing gene family. Cell Biochem. Biophys. 43(1), 95–103 (2005).

    CAS 
    PubMed 

    Google Scholar
     

  • Brisson, G. R., Malaisse-Lagae, F. & Malaisse, W. J. The stimulus-secretion coupling of glucose-induced insulin release. VII. A proposed site of action for adenosine-3’,5’-cyclic monophosphate. J. Clin. Investig. 51(2), 232–241 (1972).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Rous, S. Effect of dibutyryl cAMP on the enzymes of fatty acid synthesis and of glycogen metabolism. FEBS Lett. 12(1), 45–48 (1970).

    CAS 
    PubMed 

    Google Scholar
     

  • Geelen, M. J. & Vaartjes, W. J. Levels of cyclic 3’-5’-adenosine monophosphate (cAMP) in maintenance cultures of rat hepatocytes in response to insulin and glucagon. Lipids 12(7), 577–580 (1977).

    CAS 
    PubMed 

    Google Scholar
     

  • Stork, P. J. & Schmitt, J. M. Crosstalk between cAMP and MAP kinase signaling in the regulation of cell proliferation. Trends Cell Biol. 12(6), 258–266 (2002).

    CAS 
    PubMed 

    Google Scholar
     

  • Weissinger, E. M. et al. Inhibition of the Raf-1 kinase by cyclic AMP agonists causes apoptosis of v-abl-transformed cells. Mol. Cell Biol. 17(6), 3229–3241 (1997).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Suen, D. F., Norris, K. L. & Youle, R. J. Mitochondrial dynamics and apoptosis. Genes Dev. 22(12), 1577–1590 (2008).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Yamamoto, K. K., Gonzalez, G. A., Biggs, W. H. 3rd. & Montminy, M. R. Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB. Nature 334(6182), 494–498 (1988).

    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Chen, L. et al. Mutation of an A-kinase-anchoring protein causes long-QT syndrome. Proc. Natl. Acad. Sci. USA 104(52), 20990–20995 (2007).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Marx, S. O. et al. Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel. Science 295(5554), 496–499 (2002).

    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Bernstein, H. G. et al. Increased density of AKAP5-expressing neurons in the anterior cingulate cortex of subjects with bipolar disorder. J. Psychiatr. Res. 47(6), 699–705 (2013).

    ADS 
    PubMed 

    Google Scholar
     

  • Reissner, K. J. Proteomic analyses of PKA and AKAP signaling in cocaine addiction. Neuropsychopharmacology 38(1), 251–252 (2013).

    PubMed 

    Google Scholar
     

  • Richter, S. et al. A potential role for a genetic variation of AKAP5 in human aggression and anger control. Front. Hum. Neurosci. 5, 175 (2011).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Millar, J. K. et al. DISC1 and PDE4B are interacting genetic factors in schizophrenia that regulate cAMP signaling. Science 310(5751), 1187–1191 (2005).

    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Marquette, A. et al. ERK and PDE4 cooperate to induce RAF isoform switching in melanoma. Nat. Struct. Mol. Biol. 18(5), 584–591 (2011).

    CAS 
    PubMed 

    Google Scholar
     

  • Landis, C. A. et al. GTPase inhibiting mutations activate the alpha chain of Gs and stimulate adenylyl cyclase in human pituitary tumours. Nature 340(6236), 692–696 (1989).

    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Hinke, S. A. et al. Anchored phosphatases modulate glucose homeostasis. Embo J. 31(20), 3991–4004 (2012).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Zhang, C. L. et al. The cAMP sensor Epac2 is a direct target of antidiabetic sulfonylurea drugs. Science 325(5940), 607–610 (2009).

    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Czyzyk, T. A., Sikorski, M. A., Yang, L. & McKnight, G. S. Disruption of the RIIbeta subunit of PKA reverses the obesity syndrome of Agouti lethal yellow mice. Proc. Natl. Acad. Sci. USA 105(1), 276–281 (2008).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Calvin, H. I. et al. Induction of cortical cataracts in cultured mouse lenses with H-89, an inhibitor of protein kinase A. Curr. Eye Res. 27(5), 269–278 (2003).

    PubMed 

    Google Scholar
     

  • Chochung, Y. Differentiation therapy of cancer targeting the ri-alpha regulatory subunit of cAMP-dependent protein-kinase (review). Int. J. Oncol. 3(2), 141–148 (1993).

    CAS 
    PubMed 

    Google Scholar
     

  • Sapio, L. et al. Targeting protein kinase A in cancer therapy: An update. Excli J. 13, 843–855 (2014).

    PubMed 
    PubMed Central 

    Google Scholar
     

  • Miller, R. A. et al. Biguanides suppress hepatic glucagon signalling by decreasing production of cyclic AMP. Nature 494(7436), 256–260 (2013).

    ADS 
    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Torres, V. E. & Harris, P. C. Strategies targeting cAMP signaling in the treatment of polycystic kidney disease. J. Am. Soc. Nephrol. 25(1), 18–32 (2014).

    CAS 
    PubMed 

    Google Scholar
     

  • Wouters, E. F. et al. Effect of the phosphodiesterase 4 inhibitor roflumilast on glucose metabolism in patients with treatment-naive, newly diagnosed type 2 diabetes mellitus. J. Clin. Endocrinol. Metab. 97(9), E1720–E1725 (2012).

    CAS 
    PubMed 

    Google Scholar
     

  • Toya, Y., Schwencke, C. & Ishikawa, Y. Forskolin derivatives with increased selectivity for cardiac adenylyl cyclase. J. Mol. Cell Cardiol. 30(1), 97–108 (1998).

    CAS 
    PubMed 

    Google Scholar
     

  • Beghè, B., Rabe, K. F. & Fabbri, L. M. Phosphodiesterase-4 inhibitor therapy for lung diseases. Am. J. Respir. Crit. Care Med. 188(3), 271–278 (2013).

    PubMed 

    Google Scholar
     

  • Mulhall, A. M. et al. Phosphodiesterase 4 inhibitors for the treatment of chronic obstructive pulmonary disease: A review of current and developing drugs. Expert Opin. Investig. Drugs 24(12), 1597–1611 (2015).

    CAS 
    PubMed 

    Google Scholar
     

  • England, C. G., Ehlerding, E. B. & Cai, W. NanoLuc: A small luciferase is brightening up the field of bioluminescence. Bioconjug. Chem. 27(5), 1175–1187 (2016).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Fan, F. & Wood, K. V. Bioluminescent assays for high-throughput screening. Assay Drug Dev. Technol. 5(1), 127–136 (2007).

    CAS 
    PubMed 

    Google Scholar
     

  • Dixon, A. S. et al. NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS Chem. Biol. 11(2), 400–408 (2016).

    ADS 
    CAS 
    PubMed 

    Google Scholar
     

  • Zhang, J. H., Chung, T. D. & Oldenburg, K. R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 4(2), 67–73 (1999).

    CAS 
    PubMed 

    Google Scholar
     

  • Hwang, B. B., Engel, L., Goueli, S. A. & Zegzouti, H. A homogeneous bioluminescent immunoassay to probe cellular signaling pathway regulation. Commun. Biol. 3, 8 (2020).

    CAS 
    PubMed 

    Google Scholar
     

  • Conti, M. et al. Cyclic AMP-specific PDE4 phosphodiesterases as critical components of cyclic AMP signaling. J. Biol. Chem. 278(8), 5493–5496 (2003).

    CAS 
    PubMed 

    Google Scholar
     

  • Bobin, P. et al. Cyclic nucleotide phosphodiesterases in heart and vessels: A therapeutic perspective. Arch. Cardiovasc. Dis. 109(6–7), 431–443 (2016).

    PubMed 

    Google Scholar
     

  • Phillips, J. E. Inhaled phosphodiesterase 4 (PDE4) inhibitors for inflammatory respiratory diseases. Front. Pharmacol. 11, 259 (2020).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar
     

  • Sriram, K. & Insel, P. A. G protein-coupled receptors as targets for approved drugs: How many targets and how many drugs?. Mol. Pharmacol. 93(4), 251–258 (2018).

    CAS 
    PubMed 
    PubMed Central 

    Google Scholar